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微信小章| 产品名称： | DMS 153 |
|---|---|
| 商品货号： | TS212251 |
| Organism： | Homo sapiens, human |
| Tissue： | lung |
| Product Format： | frozen |
| Culture Properties： | monolayer of adherent clusters |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma; small cell lung cancer |
| Age： | 44 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established from metastatic cells from liver tissue taken at autopsy of a patient with small cell carcinoma of the lung. |
| Clinical Data： | The patient had received prior therapy with cytoxan and methotrexate. |
| Antigen Expression： | Leu 7 +; My23 +; CD11b + |
| Receptor Expression： | Bombesin; epidermal growth factor (EGF); complement (CR3) |
| Genes Expressed： | Adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; calcitonin gene related peptide (CGRP); oxytocin - neurophysin (OT-NP); 17 beta estradiol,Leu 7 +; My23 +; CD11b + |
| Cellular Products： | Adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; calcitonin gene related peptide (CGRP); oxytocin - neurophysin (OT-NP); 17 beta estradiol |
| Tumorigenic： | Yes |
| Effects： | Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments： | The cells express HLA class I and class II antigens. |
| Complete Growth Medium： | Waymouths MB 752/1 medium, 90%; fetal bovine serum, 10%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | OS Pettengill, G Sorenson |
| Deposited As： | Homo sapiens |
| References： | Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |