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微信小章| 产品名称： | DII 33.1 |
|---|---|
| 商品货号： | TS212252 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with carbohydrate moieties on the human insulin receptor. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with NS0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human placental insulin receptor |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human placental insulin receptor |
| Tumorigenic： | Yes |
| Effects： | Yes, in (BALB/c X SJL/J)F1 mice |
| Comments： | Animals were immunized with Wheat Germ Agglutinin bound fraction of a placental membrane extract. Spleen cells were fused with NS0 myeloma cells. The antibody reacts with carbohydrate moieties on the human insulin receptor. The antibody may cross-react with other cell surface glycoproteins. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 104 to 1 x 105 viable cells/ mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Complete growth medium described above supplemented with 10% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | S Ganguly |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Ganguly S, et al. Insulin-dependent tyrosine protein kinase: cellular distribution and copurification with the insulin receptor. Curr. Top. Cell. Regul. 27: 83-94, 1985. PubMed: 3937668 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |