微信小陈
微信小章| 产品名称： | DMS 79 |
|---|---|
| 商品货号： | TS212253 |
| Organism： | Homo sapiens, human |
| Tissue： | lung/pleural fluid |
| Product Format： | frozen |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma; small cell lung cancer |
| Age： | 65 years |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen phase |
| Karyotype： | Number of cells examined = 80; Modal Chromosome Number = 79 with a range of 65 to 84; Polyploidy Rate = 14.3% |
| Derivation： | The line was established from cells in the pleural fluid of a patient with small cell carcinoma of the lung. The patient had previously been treated with cytoxan, vincristine, methotrexate and radiation therapy. |
| Clinical Data： | 65 years
Caucasian
|
| Antigen Expression： | Leu 7; My23 |
| Receptor Expression： | epidermal growth factor (EGF) |
| Oncogene： | c-myc +; N-myc +; c-raf-1 +; H-ras +; K-ras +; N-ras +; v-fes -; v-fms - |
| Genes Expressed： | adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; corticotropin; beta endorphin; 17 beta estradiol; lipotropin; oxytocin - neurophysin (OT-NP); parathormone; somatostatin-like immunoreactivity (SRIF) |
| Cellular Products： | adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; corticotropin; beta endorphin; 17 beta estradiol; lipotropin; oxytocin - neurophysin (OT-NP); parathormone; somatostatin-like immunoreactivity (SRIF) |
| Tumorigenic： | Yes |
| Effects： | Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments： | The cell express HLA class I and class II antigens.
Derivative chromosomes observed at high frequency in 24 karyotyped metaphases included: der(2)t(2;?18)(q10;q10); del(3)?(pter-->p21::q10-->qter); del(6)(q13); der(10); add(11)(p15); der(14)t(14;17)(q10;q10); add(15)(p11); add(15)(p11)+; and del(17)(?q24). Two copies of each derivative chromosome was observed in each metaphase.
Less frequent derivative chromosomes included: del(12)?(q11q21)18/24 cells and add(13)(p11)10/24 cells. Four to eight additional marker chromosomes of unknown origin were generally detected in each cell.
Cytogenetics Comments: This is a hyper-triploid human cancer cell line with a modal chromosome number of 79.
A Y chromosome was verified by QM staining and C-banding. Normal chromosomes 10, 15 and 17 were absent.
The composite karyotype consists of 65 to 84, XY, +1, -2, der(2)t(2;?18)(q10;q10)(x2); -3, del(3)?(pter-->p21::q10-->qter)x2, -4, -5, +6, del(6)(?q15)x2,+7,+8,-9,-10,-10,-10, der(10)x2, -11, add(11)(p15)x2, -12, del(12)?(q11q21)18, -13, +14, der(14)t(14;17)(q10;q10)x2, -15, -15,- 15, der(15)x2, -16, -17, -17, 17, del(17)x2, -18, +19, -20, +21, -22cp24 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. Cell counts are approximate since the cells grow in aggregates.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10 D13S317: 11 D16S539: 12 D5S818: 10 D7S820: 9,11 THO1: 8 TPOX: 8 vWA: 18 |
| Name of Depositor： | OS Pettengill, G Sorenson |
| Deposited As： | Homo sapiens |
| References： | Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917 Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631 Pettengill OS, et al. Animal model for small cell carcinoma of the lung. Effect of immunosuppression and sex of mouse on tumor growth in nude athymic mice. Exp. Cell Biol. 48: 279-297, 1980. PubMed: 6249655 Lung Cancer 4: 155-161, 1988. |