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微信小章| 产品名称： | DPSO 114/74 |
|---|---|
| 商品货号： | TS212255 |
| Organism： | Saimiri boliviensis peruviensis, monkey, bolivian squirrel |
| Tissue： | lung |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain retrovirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | fetus |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | normal male; diploid |
| Genes Expressed： | found to release squirrel monkey retrovirus after co-cultivation with the canine thymus cell line A75 73 |
| Cellular Products： | found to release squirrel monkey retrovirus after co-cultivation with the canine thymus cell line A75 73 |
| Comments： | The line can produce a primate retrovirus and should be handled as a BIOHAZARD. |
| Complete Growth Medium： | RPMI 1640 medium - OR - Minimum essential medium (Eagle) in Earles BSS with non-essential amino acids, 90%; fetal bovine serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | SR Rangan |
| Deposited As： | Saimiri sciureus |
| References： | Scammell JG, et al. The origin of four squirrel monkey cell lines established by karyotype analysis. Cytogenet. Cell Genet. 93: 263-264, 2001. PubMed: 11528123 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |
| Cross References： | Nucleotide (GenBank) : L22859 Saimiri sciureus complement regulatory protein (CD59) mRNA, complete cds. |