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| 产品名称： | DREG200 |
|---|---|
| 商品货号： | TS212256 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | It cross-reacts with rabbit L-selectin. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human L-selectin (CD62L, LECAM, LAM-1, Leu8) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human L-selectin (CD62L, LECAM, LAM-1, Leu8) |
| Comments： | Animals were immunized with supernatant from phorbol myristic acid activated human peripheral blood leukocytes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody partially blocks lymphocyte adhesion to high enothelial venules of peripheral lymph nodes. It cross-reacts with rabbit L-selectin. |
| Complete Growth Medium： | Culture Medium: RPMI 1640 medium with 4.5 g/L glucose, 10 mM HEPES, 2 mM L-glutamine, 1 mM sodium pyruvate and 0.1 mM non-essential amino acids supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG1 |
| Name of Depositor： | EC Butcher |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Kishimoto TK, et al. Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule. Proc. Natl. Acad. Sci. USA 87: 2244-2248, 1990. PubMed: 2179952 von Andrian UH, et al. Two-step model of leukocyte-endothelial cell interaction in inflammation: distinct roles for LECAM-1 and the leukocyte beta 2 integrins in vivo. Proc. Natl. Acad. Sci. USA 88: 7538-7542, 1991. PubMed: 1715568 |