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微信小章| 产品名称： | Detroit 562 |
|---|---|
| 商品货号： | TS212258 |
| Organism： | Homo sapiens, human |
| Tissue： | pharynx: derived from metastatic site: pleural effusion |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | pharyngeal carcinoma |
| Age： | adult |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Modal number = 64; range = 58 to128 A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines |
| Clinical Data： | adult Caucasian female |
| Genes Expressed： | keratin |
| Cellular Products： | keratin |
| Virus Susceptibility： | Human poliovirus 1
Vesicular stomatitis virus |
| Comments： | The cells are positive for keratin by immunoperoxidase staining. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11,13 D13S317: 12 D16S539: 11 D5S818: 11,12 D7S820: 8,10 THO1: 8,9 TPOX: 8,10 vWA: 16 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | CS Stulberg |
| Deposited As： | Homo sapiens |
| References： | Peterson WD Jr., et al. Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc. Soc. Exp. Biol. Med. 128: 772-776, 1968. PubMed: 5668122 Peterson WD Jr., et al. A permanent heteroploid human cell line with type B glucose-6-phosphate dehydrogenase. Proc. Soc. Exp. Biol. Med. 136: 1187-1191, 1971. PubMed: 5554463 |