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微信小章| 产品名称： | Detroit 529 |
|---|---|
| 商品货号： | TS212260 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Down syndrome |
| Age： | 2.5 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | number = 48; trisomic for X; trisomic for a group G chromosome; Down syndrome |
| Clinical Data： | female Caucasian 2.5 years |
| Virus Susceptibility： | Human poliovirus 1
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| Comments： | The cells have a finite life expectancy of 30 serial passages from the tissue of origin. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: 5% Carbon dioxide (CO2) |
| STR Profile： | Amelogenin: X CSF1PO: 11,13 D13S317: 11,13 D16S539: 13,14 D5S818: 12,13 D7S820: 10 THO1: 8,9 TPOX: 8,11 vWA: 18,19 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | CS Stulberg |
| Deposited As： | Homo sapiens |
| Passage History： | The cells have a finite life expectancy of 30 serial passages from the tissue of origin. |
| Year of Origin： | October, 1963 |
| References： | Hart ZH, et al. A sex chromatin negative individual with chromosomes (XO) plus a persistent centric fragment. J. Pediatr. 66: 120-123, 1965. PubMed: 14253578 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |