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微信小章| 产品名称： | DHFR-G8 |
|---|---|
| 商品货号： | TS212266 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Cell Type： | fibroblast fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Strain： | NIH/Swiss |
| Applications： | The DHFR-G8 cell line was produced by M.C. Hung, et al. This cell line was developed from one methotrexate resistant colony by selection in 0.6 uM methotrexate and 10% dialyzed calf serum. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The DHFR-G8 cell line was produced by M.C. Hung, et al., in 1986 from the NIH/3T3 mouse fibroblast cell line which was cotransfected with the cNEU-p clone and the pSV2-DHFR plasmid. This cell line was developed from one methotrexate resistant colony by selection in 0.6 uM methotrexate and 10% dialyzed calf serum. These cells contain 50-100 copies of the cNeu-P (normal rat cosmid DNA) and produce approximately 4 x 10(5) molecules of encoded p185 protein per cell. |
| Comments： | The DHFR-G8 cell line was produced by M.C. Hung, et al., in 1986 from the NIH/3T3 mouse fibroblast cell line which was cotransfected with the cNEU-p clone and the pSV2-DHFR plasmid. This cell line was developed from one methotrexate resistant colony by selection in 0.6 uM methotrexate and 10% dialyzed calf serum. These cells contain 50-100 copies of the cNeu-P (normal rat cosmid DNA) and produce approximately 4 x 10(5) molecules of encoded p185 protein per cell. |
| Complete Growth Medium： | Dulbeccos modified Eagles Medium with 4.5 g/L glucose and 300 nM methotrexate, 90%; bovine calf serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa04th edition, published by Wiley-Liss, N.Y., 2000. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | RA Weinberg |
| Deposited As： | Mus musculus |
| Year of Origin： | 1986 |
| References： | Hung MC, et al. Molecular cloning of the neu gene: Absence of gross structural alteration in oncogenic alleles. Proc. Natl. Acad. Sci. USA 83: 261-264, 1986. PubMed: 3001730 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |