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微信小章| 产品名称： | Dede |
|---|---|
| 商品货号： | TS212270 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | lung |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | female |
| Clinical Data： | female |
| Virus Resistance： | poliovirus 1 |
| Complete Growth Medium： | McCoyx92s 5a medium (modified) with 1.5 mM L-glutamine adjusted to contain 2.2 g/L sodium bicarbonate, 80%; fetal bovine serum, 20%
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and allow culture to sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
Note: If necessary, the serum can be reduced to 10%. |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | TC Hsu |
| Deposited As： | Cricetulus griseus |
| References： | Stich HF, et al. Viruses and mammalian chromosomes. I. Localization of chromosome aberrations after infection with Herpes simplex virus. Virology 22: 439-445, 1964. PubMed: 14171598 Rapp F, Hsu TC. Viruses and mammalian chromosomes. IV. Replication of Herpes simplex virus in diploid Chinese hamster cells. Virology 25: 401-411, 1965. PubMed: 14328610 Stich HF, et al. Viruses and mammalian chromosomes; chromosome aberrations by human adenovirus type 12. Exp. Cell Res. 34: 400-403, 1964. PubMed: 14165598 |
| Cross References： | Nucleotide (GenBank) : X86458 C.griseus mRNA for serotonin receptor 1B. Nucleotide (GenBank) : X61958 C.longicaudatus mRNA for thrombin receptor. Nucleotide (GenBank) : X53791 Chinese hamster mRNA for 5-HT type 2 serotonin receptor. |