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微信小章| 产品名称： | Dempsey |
|---|---|
| 商品货号： | TS212274 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Klinefelter syndrome |
| Age： | 1 year 10 months |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | model number = 49; range = 41 to 95 Stability graph shows 100% stability in the stemline number (49). Karyotypes of cells with stemline number of chromosomes were stable with all cells having three extra chromosomes in the X, 6-12 group. |
| Clinical Data： | male Caucasian 1 year 10 months |
| Virus Susceptibility： | Human poliovirus 1
Vesicular stomatitis virus |
| Comments： | Klinefelter Syndrome - XXXXY. Senesces after approximately 30 passages. |
| Complete Growth Medium： | McCoyx92s 5a medium (modified) with 1.5 mM L-glutamine adjusted to contain 2.2 g/L sodium bicarbonate, 80%; fetal bovine serum, 20%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.xa0xa0 |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11 D16S539: 11,12 D5S818: 11,13 D7S820: 8,12 THO1: 8,9.3 TPOX: 8 vWA: 17,18 |
| Isoenzymes： | G6PD, B |
| Population Doubling Capacity： | Senesces after approximately 30 passages |
| Name of Depositor： | TC Hsu |
| Deposited As： | Homo sapiens |
| Year of Origin： | April, 1964 |
| References： | Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475 Hwu TC, Lockhart LH. The beginning and the terminal stages of DNA synthesis of human cells with an XXXXY constitution. Hereditas 52: 320-324, 1965. PubMed: 5889982 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |