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| 产品名称： | Daoy |
|---|---|
| 商品货号： | TS212279 |
| Organism： | Homo sapiens, human |
| Tissue： | brain/cerebellum |
| Product Format： | frozen |
| Morphology： | polygonal |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | desmoplastic cerebellar medulloblastoma |
| Age： | 4 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a hypertetraploid human cell line with a modal number between 93 and 99. The frequency of cells with higher ploidies is 2.0%. However, since the stemline chromosome number is high, the estimate for the polyploidy is tentative. Thirteen or more marker chromosomes were common to all cells. Of these, many had two to four copies per cell. Among the markers were: t(1q5q), t(13q;?), 15p+, 7q+, der(9)t(3;9)(p21;q34) and eight others. In most cells, the 15p+ has three copies and der(9) has four copies.Some cells have del(1)(p11). Normal N12, N14, N15 and N19 tend to have four or more copies per cell. There are two normal X chromosomes in most cells, but there is no detectable normal Y. |
| Images： |  |
| Derivation： | The Daoy cell line was established in 1985 by P. F Jacobsen of the Royal Perth Hospital in Western Australia. The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy. |
| Clinical Data： | Caucasian male The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy. 4 years |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (The cells form serially transplantable intercranial and subcutaneous tumors.) |
| Comments： | Although the original tumor had characteristics of both neuronal and glial differentiation, these were not retained by the cell line. Treatment of the cells with dibutyryl cyclic amp (cAMP) does not induce expression of those characteristics as measured by staining for S100 (S-100) protein and glial fibrillary acidic proteins (GFAP). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 13,14 D16S539: 10 D5S818: 11,13 D7S820: 8,10 THO1: 9 TPOX: 8,10 vWA: 14,20 |
| Isoenzymes： | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 1 Me-2, 1 PGM1, 2 PGM3, 1-2 |
| Population Doubling Time： | 34 hrs |
| Name of Depositor： | HS Friedman |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1985 |
| References： | He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373 Jacobsen PF, et al. Establishment of a human medulloblastoma cell line and its heterotransplantation into nude mice. J. Neuropathol. Exp. Neurol. 44: 472-485, 1985. PubMed: 2993532 Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024 The cells form serially transplantable intercranial and subcutaneous tumors. |