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| 产品名称： | DB |
|---|---|
| 商品货号： | TS212282 |
| Organism： | Homo sapiens, human |
| Tissue： | Ascites |
| Cell Type： | B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | large cell lymphoma |
| Age： | 45 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The DB cell line was established by Walter J. Urba and Dan L. Longo from ascites fluid from a patient with a diffuse large cell lymphoma. |
| Clinical Data： | 45 years
Caucasian
male
|
| Antigen Expression： | CD19 +; CD20 +; CD22 +; Hle-1 +; CD21 -; CD25 - |
| Cellular Products： | immunoglobulin |
| Comments： | The cells do not express HLA-DR, HLA-DQ, IgM , IgD or T cell rantigen receptor.
Exposure of DB cells to phorbol esters (PMA or PdBu) that activate protein kinase C results in profound growth inhibition.
They are reported to be negative for the Epstein-Barr virus genome.
The cells were cured of a prior mycoplasma contamination by a 21 day treatment with BM-cycline. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL. Maintain cultures at a cell concentration between 5 x 105 and 2 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL. Medium Renewal: 2 to 3 times weekly. |
| Cryopreservation： | Freeze Medium: Complete growth medium, 90%; DMSO, 10%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 11,12 D16S539: 11 D5S818: 11 D7S820: 11 THO1: 6,8 TPOX: 8 vWA: 15,16 |
| Isotype： | IgG; lambda light chain |
| Name of Depositor： | M Beckwith |
| Deposited As： | Homo sapiens |
| References： | Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723 |