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微信小章| 产品名称： | Daudi |
|---|---|
| 商品货号： | TS212283 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells contain Herpesvirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Age： | 16 years |
| Gender： | male |
| Ethnicity： | Black |
| Applications： | Daudi is a well characterized B lymphoblast cell line which has been employed extensively in studies of mechanisms of leukemogenesis. The cell line is also a suitable transfection host |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Male human karyotype with stemline number of 46. The karyotype is diploid in 66% of the cells and is stable within the stemline. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Derivation： | The Daudi line was derived from a 16-year-old Black male with Burkitts lymphoma by E. Klein and G. Klein in May, 1967. |
| Clinical Data： | 16 years
Black
male |
| Receptor Expression： | complement, expressed Fc, expressed |
| Tumorigenic： | Yes, in nude mice; forms colonies in agarose |
| Effects： | Yes, in agarose Yes, in nude mice |
| Comments： | The cells are negative for beta-2-microglobulin. They are positive for EBNA, VCA and Surface immunoglobulin (sIg+). ATCC confirmed this cell line is positive for the presence of EBV viral DNA sequences via PCR. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 to 5 x 105 viable cells/mL.xa0Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.xa0
Interval: Maintain cell density between 3 x 105 and 2 to 3 x 106 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 12 D13S317: 11,12 D16S539: 10,12 D5S818: 8,13 D7S820: 8,10 THO1: 6,7 TPOX: 8,11 vWA: 15,17 |
| Isoenzymes： | G6PD, B |
| Isotype： | IgM |
| Name of Depositor： | G Klein |
| Deposited As： | Homo sapiens |
| Year of Origin： | May, 1967 |
| References： | Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523 Klein E, et al. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 28: 1300-1310, 1968. PubMed: 4174339 Huber C, et al. Surface receptors on human haematopoietic cell lines. Clin. Exp. Immunol. 25: 367-378, 1976. PubMed: 963908 Nilsson K, et al. Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer 19: 337-344, 1977. PubMed: 14896 Gao Y, et al. Induction of an exceptionally high-level, nontranslated, Epstein-Barr virus-encoded polyadenylated transcript in the uurkitts lymphoma line Daudi. J. Virol. 71: 84-94, 1997. PubMed: 8985326 Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019 Yamaguchi Y, et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055 Lewis JA, et al. Inhibition of mitochondrial function by interferon. J. Biol. Chem. 271: 13184-13190, 1996. PubMed: 8662694 Montoya JG, et al. Human CD4+ and CD8+ T lymphocytes are both cytotoxic to Toxoplasma gondii-infected cells. Infect. Immun. 64: 176-181, 1996. PubMed: 8557337 |
| Cross References： | Nucleotide (GenBank) : U07990 Human Burkitts lymphoma immunoglobulin kappa light chain mRNA, partial cds. Nucleotide (GenBank) : U07987 Human Burkitts lymphoma immunoglobulin IgM heavy chain Fd fragment mRNA, partial cds. |