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微信小章| 产品名称： | D341 Med |
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| 商品货号： | TS212288 |
| Organism： | Homo sapiens, human |
| Tissue： | brain/cerebellum |
| Cell Type： | Medulloblastoma |
| Product Format： | frozen |
| Morphology： | spheroid |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | medulloblastoma |
| Age： | 3.5 years |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a hyperdiploid (modal number = 49; range = 44 to 50) human cell line with higher ploidies occurring with a frequency of 8.5%. Four marker chromosomes are present in all cells. They are: 11HSR, i(17q), der(22)t(1;22)(q12;p12) and 8q-. There are three copies each of N6 and N18, and single copies each of N11, N17 and N22. The single X chromosome was structurally normal, and the presence of the Y chromosome was confirmed by fluorescence microsopy. |
| Images： |  |
| Derivation： | The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma. |
| Clinical Data： | The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma. male |
| Genes Expressed： | The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody. |
| Cellular Products： | neurofilament proteins |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms serially transplantable intercranial and subcutaneous tumors Yes, in soft agar |
| Comments： | The cells produce tumors in nude mice. The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody. The c-myc oncogene is amplified in D341 Med cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium. Cells grow mostly as floating aggregates of irregular-shaped cell clusters with some attached cells. Clusters are alive while single cells have very low viability. Attached cells can be shaken loose.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: culture medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,13
D16S539: 12,14
D5S818: 11,12
D7S820: 9,13
THO1: 6,9.3
TPOX: 8,11
vWA: 17,18 |
| Population Doubling Time： | 37 hrs |
| Name of Depositor： | HS Friedman |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1988 |
| References： | Friedman HS, et al. Phenotypic and genotypic analysis of a human meduloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am. J. Pathol. 130: 472-484, 1988. PubMed: 3279793 He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373 Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024 |