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| 产品名称： | Cyt c-/- |
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| 商品货号： | TS212299 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 8-9 days gestation |
| Applications： | The Cyt c-/- cell line provides a unique resource of mammalian cells lacking cytochrome c. |
| Derivation： | The Cyt c -/- cell line was derived from a cytochrome c null mouse embryo at E8/E9 (8-9 days gestation) prior to embryonic death. |
| Comments： | Murine embryos devoid of cytochrome c die in utero by midgestation.xa0
Cytochrome c released from mitochondria has been proposed to be an essential component of an apoptotic pathway responsive to DNA damage and other forms of cell stress.
The Cyt c -/- cells are devoid of cytochrome c and are resistant to various types of apoptotic stimuli.
They show reduced caspase-3 activation and are resistant to the proapoptotic effects of UV irradiation, serum withdrawal, and staurosporine. They demonstrate increased sensitivity to cell death signals triggered by TNFalpha.
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| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with: 25 mM HEPES, 0.1 mM nonessential amino acids, an additional 2 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 0.05 mg/ml uridine, 500-1000 U/ml mouse leukemia inhibitory factor (LIF) and 20% fetal bovine serum
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 3 days |
| Cryopreservation： | Cell Culture Freezing Medium, 100% (GIBCO-BRL, Cat. No. 11101) |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | RS Williams |
| Deposited As： | mouse |
| References： | Li K, et al. Cytochrome c deficiency causes embryonic lethality and attenuates stress-induced apoptosis. Cell 101: 389-399, 2000. PubMed: 10830166 |