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微信小章| 产品名称： | CTX TNA2 |
|---|---|
| 商品货号： | TS212311 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | brain, cortex |
| Cell Type： | astrocyte, type 1 phenotype |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 CELLS CONTAIN PAPOVAVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | neonate |
| Strain： | Sprague-Dawley |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The CTX TNA2 cell line was established from primary cultures of type 1 astrocytes from brain frontal cortex tissue of 1 day old rats. The cultures were transfected 3 days after initial plating with a DNA construct containing the oncogenic early region of SV40 under the transcriptional control of the human GFAP promoter (pGFA-SV-Tt) and pPGK-neo which contains the murine phosphoglycerate kinase gene promoter. The transfectants were selected with G418 and cloned. |
| Genes Expressed： | Alpha 2 macroglobulin; transferrin |
| Cellular Products： | alpha 2 macroglobulin; transferrin |
| Tumorigenic： | No |
| Effects： | No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments： | The cells retain characteristics consistent with the phenotype of type 1 astrocytes.
About 20% of the cells have glial fibrillary acidic protein (GFAP) immunoreactivity. The cells have a high affinity uptake mechanism for gamma aminobutyric acid (GABA) that is inhibitable by beta alanine. The cells produce alpha 2 macroglobulin in amounts similar to those found in primary astrocytes but produce transferrin in much lesser amounts. This line does not produce proenkephalin A, does not express the O4 or A2B5 epitopes characteristic of type 2 astrocytes, and does not express galactocerebroside. SV40 T-antigen was found in the nuclei of over 95% of the cells examined by immunostaining. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Name of Depositor： | CF Deschepper |
| Deposited As： | Rattus sp. |
| References： | Radany EH, et al. Directed establishment of rat brain cell lines with the phenotypic characteristics of type 1 astrocytes. Proc. Natl. Acad. Sci. USA 89: 6467-6471, 1992. PubMed: 1378628 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |