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微信小章| 产品名称： | CV-1 |
|---|---|
| 商品货号： | TS212314 |
| Organism： | Cercopithecus aethiops |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Age： | 141 days |
| Gender： | male |
| Applications： | efficacy testing
transfection host
viruscide testing |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a pseudodiploid, male African green monkey cell line. The modal chromosome number was 60, occurring in 48% of cells, and the rate of polyploidy was at 4.4%. Only a few markers were found., Of these M1, a probable deleted N11, was found in all cells examined; M3 of unknown origin was in some cells; and the remaining 2 to 3 others of unknown origins were found only once., N11 was uniformly single copied, and N16 was also single copied in most cells. Both X and Y chromosomes were also detected in every cell. |
| Derivation： | The CV-1 cell line was derived from the kidney of a male adult African green monkey by F.C. Jensen, et al. in March, 1964 for use in Rous sarcoma virus transformation studies. |
| Clinical Data： | adult African green monkey
male
|
| Virus Susceptibility： | Human poliovirus 1
Herpes simplex virus Eastern equine encephalitis virus , Eastern equine encephalitis virus Western equine encephalitis virus , Western equine encephalitis virus California encephalitis virus , Melao virus SV40 virus |
| Comments： | This cell line is a suitable host for transfection, especially by SV40 vectors. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week
|
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | FC Jensen |
| Deposited As： | Cercopithecus aethiops |
| Year of Origin： | 1964 |
| References： | Jensen FC, et al. Infection of human and simian tissue cultures with Rous Sarcoma Virus. Proc. Natl. Acad. Sci. USA 52: 53-59, 1964. PubMed: 14192657 Kit S, et al. Enhanced thymidine kinase activity following infection of green monkey kidney cells by simian adenoviruses, simian papovavirus SV40, and an adenovirus-SV40 "hybrid". Virology 27: 453-457, 1965. PubMed: 4285109 Deleersnyder V, et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. PubMed: 8985401 Rhoades KL, et al. Synergistic up-regulation of the myeloid-specific promoter for the macrophage colony-stimulating factor receptor by AML1 and the t(8;21) fusion protein may contribute to leukenogenesis. Proc. Natl. Acad. Sci. USA 93: 11895-11900, 1996. PubMed: 8876234 Halford WP, et al. Mechanisms of herpes simplex virus type 1 reactivation. J. Virol. 70: 5051-5060, 1996. PubMed: 8764012 Wu TT, et al. Evidence that two latency-associated transcripts of herpes simplex virus type 1 are nonlinear. J. Virol. 70: 5962-5967, 1996. PubMed: 8709218 Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256 Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 Planelles V, et al. Vpr-induced cell cycle arrest is conserved among primate lentiviruses. J. Virol. 70: 2516-2524, 1996. PubMed: 8642681 Hill JM, et al. In vivo epinephrine reactivation of ocular herpes simplex virus type 1 in the rabbit is correlated to a 370-base-pair region located between the promoter and the 5 end of the 2.0-kilobase latency-associated transcript. J. Virol. 70: 7270-7274, 1996. PubMed: 8794381 Dubuisson J, Rice CM. Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin. J. Virol. 70: 778-786, 1996. PubMed: 8551615 Biardi L, Krisans SK. Compartmentalization of cholesterol biosynthesis. J. Biol. Chem. 271: 1784-1788, 1996. PubMed: 8576183 Pei L. Identification of a negative glucocorticoid response element in the rat type 1 vasoactive intestinal polypeptide receptor gene. J. Biol. Chem. 271: 20879-20884, 1996. PubMed: 8702844 Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651 Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02. Standard Test Method for Evaluation of Handwashing Formulation for Virus-Eliminating Activity using the Entire Hand. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2011-99. Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02. |
| Cross References： | Nucleotide (GenBank) : U83324 Cercopithecus aethiops CC chemokine receptor-5 (CCR5) gene, clone 1, complete cds. Nucleotide (GenBank) : U83325 Cercopithecus aethiops CC chemokine receptor-5 (CCR5) gene, clone 2, complete cds. |