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微信小章| 产品名称： | CPAE |
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| 商品货号： | TS212319 |
| Organism： | Bos taurus, cow |
| Tissue： | pulmonary artery/endothelium |
| Cell Type： | endothelial |
| Product Format： | frozen |
| Morphology： | endothelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain Bovine Viral Diarrhea Virus (BVDV)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | normal female; diploid; stable |
| Images： |  |
| Derivation： | CPAE is an endothelial cell line derived from the main stem pulmonary artery of a young cow (Bos taurus). This line was initiated in January, 1979 by P. Del Vecchio from artery lumen scrapings dispersed by enzyme treatment. |
| Clinical Data： | female |
| Genes Expressed： | positive for angiotensin converting enzyme |
| Cellular Products： | positive for angiotensin converting enzyme |
| Comments： | Subsequent to the publication of the sixth edition of the ATCC catalog, tests for bovine diarrhea virus (BVD) have indicated that CPAE cells test positive for BVD viral antigen. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week
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| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C
Atmosphere:xa095% air; 5% CO2 |
| Name of Depositor： | PJ Del Vecchio |
| Deposited As： | Bos taurus |
| Year of Origin： | 1979 |
| References： | Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 Girard JP, Springer TA. Modulation of endothelial cell adhesion by hevin, an acidic protein associated with high endothelial venules. J. Biol. Chem. 271: 4511-4517, 1996. PubMed: 8626806 |