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微信小章| 产品名称： | Con A - C1 - VICK |
|---|---|
| 商品货号： | TS212321 |
| Organism： | Gallus gallus, chicken |
| Tissue： | spleen |
| Cell Type： | T lymphocyte; transformed with reticuloendotheliosis virus (ATCC VR-770 |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, multicell aggregates, clusters in suspension |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 42 week old |
| Gender： | female |
| Applications： | Two immortal cell lines were established, CON A-C1-VICK (TS212321) and ConA-B1-VICK (ATCC CRL-12357). Both cell lines produce granulocyte colony stimulating factor (G-CSF). When cultured in vitro, the cell lines produce and secrete immune lymphokines that may be administered to fowl to increase their resistance to infections. The cell lines produce lymphokines which, following administration into either 18 day old chick embryos or day of hatch chicks, prevents extraintestinal Salmonella infection. Neither the lymphokine or cell line induces viral pathogenesis in chickens. For lymphokine production, remove cells from maintenance medium, wash in serum-free RPMI, culture at 5 x 10 exp6 cells/ml in serum-free RPMI containing 0.0075 mg/ml Concanavalin A (ConA) for 72 hours. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleens were harvested and T cells were isolated. |
| Clinical Data： | female |
| Genes Expressed： | granulocyte colony stimulating factor (G-CSF) |
| Cellular Products： | granulocyte colony stimulating factor (G-CSF) |
| Comments： | Animals were immunized with Salmonella enteritidis. Spleens were harvested and T cells were isolated. T cells were incubated with Concanavalin A and subsequently transformed with Avian reticuloendotheliosis virus (REV-T with CSV)(ATCC VR-770). Two immortal cell lines were established, CON A-C1-VICK (TS212321) and ConA-B1-VICK (ATCC CRL-12357). Both cell lines produce granulocyte colony stimulating factor (G-CSF). When cultured in vitro, the cell lines produce and secrete immune lymphokines that may be administered to fowl to increase their resistance to infections. The cell lines produce lymphokines which, following administration into either 18 day old chick embryos or day of hatch chicks, prevents extraintestinal Salmonella infection. Neither the lymphokine or cell line induces viral pathogenesis in chickens. For lymphokine production, remove cells from maintenance medium, wash in serum-free RPMI, culture at 5 x 10 exp6 cells/ml in serum-free RPMI containing 0.0075 mg/ml Concanavalin A (ConA) for 72 hours. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL. Medium Renewal: 2 to 3 times weekly |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | USDA/ARS |
| Deposited As： | chicken |
| U.S. Patent Number： | |
| References： | Kogut MH, et al. Method to produce granulocyte colony stimulating factor from immortalized avian T lymphocytes and method to produce immortalized cells. US Patent 5,691,200 dated Nov 25 1997 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |