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微信小章| 产品名称： | COS-7 |
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| 商品货号： | TS212323 |
| Organism： | Cercopithecus aethiops |
| Tissue： | kidney |
| Cell Type： | SV40 transformed |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells Contain SV-40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The line was derived from the CV-1 cell line (ATCC CCL-70 ) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen. |
| Genes Expressed： | T antigen |
| Cellular Products： | T antigen |
| Comments： | This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40°C, and supports the replication of pure populations of SV40 mutants with deletions in the early region. The line was derived from the CV-1 cell line (ATCC CCL-70 ) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | Y Gluzman |
| Deposited As： | Cercopithecus aethiops |
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