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微信小章| 产品名称： | CT6-1D7 |
|---|---|
| 商品货号： | TS212327 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody recognizes the cytoplasmic region of both Fc gamma RI a1 and Fc gamma RI b2 in immunoadsorption assays and Western blot analysis but Fc gamma a1 is better recognized than Fc gamma b2. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the cytoplasmic tail of the human high affinity Fc receptor for IgG (Fc gamma RI) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the cytoplasmic tail of the human high affinity Fc receptor for IgG (Fc gamma RI) |
| Comments： | Animals were immunized with an 18-residue peptide in the cytoplasmic domain near the plasma membrane of FcgammaRIa1. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody recognizes the cytoplasmic region of both Fc gamma RI a1 and Fc gamma RI b2 in immunoadsorption assays and Western blot analysis but Fc gamma a1 is better recognized than Fc gamma b2. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.
To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 2 x 105 and 8 x 105 viable cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | CL Anderson |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |