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微信小章| 产品名称： | COLO 201 |
|---|---|
| 商品货号： | TS212332 |
| Organism： | Homo sapiens, human |
| Tissue： | colon; derived from metastatic site: ascites |
| Product Format： | frozen |
| Morphology： | bipolar, slightly refractile, fibroblast-like cell |
| Culture Properties： | suspension, with some loosely adherent cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dukes type D, colorectal adenocarcinoma |
| Age： | 70 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | The stemline chromosome number is hypertriploid and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) were common in monosomic, disomic and trisomic condition although most of these markers were monosomic. |
| Derivation： | This cell line was derived from tissue from the same patient as COLO 205 (ATCC CCL-222). |
| Clinical Data： | 70 years
Caucasian
male |
| Oncogene： | myc +; ras + ; myb + ; fos +; sis +; p53 +; abl -; ros -; src - |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments： | The line is negative for CSAp (CSAp-) and CEA. This cell line is tumorigenic in mice.xa0Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a 0.25% trypsin, 0.53 mM EDTA solution. Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: 1 to 2 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 10,12 D16S539: 12,13 D5S818: 10,13 D7S820: 9,10 THO1: 8,9 TPOX: 11 vWA: 15 |
| Isoenzymes： | ES-D, 1-2 G6PD, B PEP-D, 1 PGD, A PGM1, 1-2 PGM3, 1-2 |
| Name of Depositor： | GE Moore |
| Deposited As： | Homo sapiens |
| References： | Semple TU, et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251 Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 |