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微信小章| 产品名称： | COLO 205 |
|---|---|
| 商品货号： | TS212333 |
| Organism： | Homo sapiens, human |
| Tissue： | colon; derived from metastatic site: ascites |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | mixed, adherent and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dukes type D, colorectal adenocarcinoma |
| Age： | 70 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | The stemline chromosome number is hypertriploid and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) were common in monosomic, disomic and trisomic condition although most of these markers were monosomic. |
| Derivation： | This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen. |
| Clinical Data： | 70 years Caucasian male The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen. |
| Genes Expressed： | carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10),The cells are positive for keratin by immunoperoxidase staining. |
| Cellular Products： | carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10) |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments： | The line was derived from tissue from the same patient as COLO 201 (ATCC CCL-224). The cells are CSAp negative (CSAp-). The cells are positive for keratin by immunoperoxidase staining.
COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen. xa0 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cells grow loosely attached and in suspension. Shake flask, retain the floating cells by transfering them into a centrifuge tube. Cells that remain attached may be removed using a standard trypsinization protocol (see below) and combined with the retained floating cells. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 10,12 D16S539: 12,13 D5S818: 10,13 D7S820: 9,10 THO1: 8,9 TPOX: 11 vWA: 15 |
| Isoenzymes： | ES-D, 1-2 G6PD, B PEP-D, 1 PGD, A PGM1, 1-2 PGM3, 1-2 |
| Name of Depositor： | GE Moore |
| Deposited As： | Homo sapiens |
| References： | Semple TU, et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251 Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757 Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 Bjork P, et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241, 1993. PubMed: 7693697 |
| Cross References： | Nucleotide (GenBank) : AB012142 Homo sapiens hCAP1a mRNA for mRNA capping enzyme, complete cds. Nucleotide (GenBank) : AB012143 Homo sapiens hCAP1b mRNA for mRNA capping enzyme, complete cds. |