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购买须知
| 产品名称： | COLO 829 |
|---|---|
| 商品货号： | TS212335 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Melanoma |
| Age： | 45 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The COLO 829 cell line was established by G.E. Moore from a biopsy specimen taken from a patient prior to chemotherapy for metastatic melanoma.
|
| Clinical Data： | 45 years Caucasian male |
| Comments： | A cell line was also produced from peripheral blood B cells, and is available as COLO 829BL (ATCC CRL-1980)
Early passages of COLO 829 still contain a small percentage of melanin producing cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of Trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 11,12 D16S539: 13 D5S818: 11 D7S820: 8,10 THO1: 10 TPOX: 8,11 vWA: 17,19 |
| Name of Depositor： | GE Moore |
| Deposited As： | Homo sapiens |
| Passage History： | Early passages of COLO 829 still contain a small percentage of melanin producing cells. |