微信小陈
微信小章
产品简介
购买须知
| 产品名称： | clone 9 |
|---|---|
| 商品货号： | TS212341 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | liver |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 4 weeks |
| Gender： | male |
| Strain： | Sprague-Dawley |
| Applications： | This cellxa0line has been used for studies of in vitro carcinogenesis and is useful in clonal assays for screening sera and other nutritional supplements. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Clone 9 (K-9) is an epithelial cell line isolated in 1968 from normal liver taken from a young male rat. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | ME Kaighn |
| Deposited As： | Rattus sp. |
| Year of Origin： | 1968 |
| References： | . Gene expression and carcinogenesis in cultured liver. New York: Academic Press; 1975. Weinstein IB, et al. Growth and structural properties of epithelial cell cultures established from normal rat liver and chemically induced hepatomas. Cancer Res. 35: 253-263, 1975. PubMed: 162864 |