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微信小章| 产品名称： | Clone M-3 Cloudman S91 melanoma |
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| 商品货号： | TS212342 |
| Organism： | Mus musculus, mouse |
| Tissue： | skin |
| Cell Type： | melanocyte |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | melanoma |
| Gender： | male |
| Strain： | DBA |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Stemline number is hypertetraploid. Karyotype is stable within stemline number. Marker chromosomes: A medium size chromosome with a submedian centromere and a smaller chromosome with a median centromere., The remaining 81 chromosomes have terminal centromeres, the first one is larger than normal. A minute chromosome was noted in 20% of the cells. |
| Derivation： | Clone M-3, a melanin-producing cell line was adapted to cell culture by Y. Yasumura, A.H. Tashjian and G. Sato from a Cloudman S91 melanoma in a (C X DBA) F1 male mouse obtained from the Jackson Memorial Laboratory, Bar Harbor, Maine. |
| Clinical Data： | male |
| Genes Expressed： | melanin |
| Cellular Products： | melanin |
| Tumorigenic： | Yes |
| Effects： | Yes, in C57BL mice (Melanotic tumors developed after inoculation of 10(4) cells in 5/6 mice.) |
| Virus Susceptibility： | Herpes simplex virus
Vaccinia virus Pseudorabies virus Vesicular stomatitis, Glasgow (Indiana) Vesicular stomatitis, Orsay (Indiana) |
| Virus Resistance： | poliovirus 1 |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every other day
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| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | G Sato |
| Deposited As： | Mus musculus |
| References： | Yasamura Y, et al. Establishment of four functional, clonal strains of animal cells in culture. Science 154: 1186-1189, 1966. PubMed: 4288399 Schmidt W, et al. Cell-free tumor antigen peptide-based cancer vaccines. Proc. Natl. Acad. Sci. USA 94: 3262-3267, 1997. PubMed: 9096381 |