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| 产品名称： | CMH1a |
|---|---|
| 商品货号： | TS212346 |
| Organism： | Mus musculus, mouse |
| Tissue： | mammary gland |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Papilloma, hepatitis |
| Strain： | RIII |
| Applications： | This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg. HBsAg production is under the control of the metallothionine promoter. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg. |
| HeLa Markers： | N |
| Genes Expressed： | surface antigen sAg |
| Comments： | This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg. HBsAg production is under the control of the metallothionine promoter. The construct contains the entire BPV genome and the hepatitis B sAg structural gene inserted within the MT gene between the promoter and the rest of the gene. A BamHI/SalI fragment of pRF300 (containing hepatitis B sAg and MT-1 promoter) was ligated to a BamHI/SalI fragment of pB2-2 (containing the entire BPV genome and pBR sequences) to create pRB-1. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:15 is recommended Medium Renewal: 2 to 3 times per week Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature (or 37C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense, into new flasks. |
| Cryopreservation： | fetal bovine serum, 90%; glycerol, 10% |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | Integrated Genetics, Inc. |
| Deposited As： | Mus musculus |
| U.S. Patent Number： | |
| References： | Fitts RA, et al. Vector encoding hepatitis B surface antigen. US Patent 4,496,898 dated Sep 29 1987 |