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微信小章| 产品名称： | CL18/6 |
|---|---|
| 商品货号： | TS212350 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with canine intercellular adhesion molecule 1 (ICAM-1). Antibody produced by CL18/6 (TS212350), but not CL18/1 (ATCC CRL-2517), blocks adherence of canine neutrophils to lipopolysaccharide (LPS) stimulated canine jugular vein endothelial cells. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. Antibody produced by CL18/6 (TS212350), but not CL18/1 (ATCC CRL-2517), blocks adherence of canine neutrophils to lipopolysaccharide (LPS) stimulated canine jugular vein endothelial cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against canine intracellular adhesion molecule 1 (ICAM-1). |
| Cellular Products： | immunoglobulin; monoclonal antibody; against canine intracellular adhesion molecule 1 (ICAM-1). |
| Comments： | Animals were immunized with canine endothelial cells that had been stimulated with lipopolysaccharide (LPS). Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody reacts with canine intercellular adhesion molecule 1 (ICAM-1). Antibody produced by CL18/6 (TS212350), but not CL18/1 (ATCC CRL-2517), blocks adherence of canine neutrophils to lipopolysaccharide (LPS) stimulated canine jugular vein endothelial cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cell density between 5 x 104 and 1 x 106 viable cells/mL. Medium Renewal:xa0 Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | C Smith |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Smith CW, et al. Adherence of neutrophils to canine cardiac myocytes in vitro is dependent on intercellular adhesion molecule-1. J. Clin. Invest. 88: 1216-1223, 1991. PubMed: 1680878 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |