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微信小章| 产品名称： | Cl. Ly1+2-/9 |
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| 商品货号： | TS212354 |
| Organism： | Mus musculus, mouse |
| Tissue： | spleen |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | C57BL/6-TL+ |
| Applications： | This is a cloned T cell line derived from C57BL/6TL+ spleen cells. The cells produce IL-3, TCGF2, MCGF2 and BSF-1. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | This is a cloned T cell line derived from C57BL/6TL+ spleen cells. |
| Genes Expressed： | interleukin-3 (interleukin 3, IL-3); T cell growth factor 2 (TCGF2); mast cell growth factor 2 (MCGF2); B cell stimulating factor 1 (BSF-1) |
| Cellular Products： | interleukin-3 (interleukin 3, IL-3); T cell growth factor 2 (TCGF2); mast cell growth factor 2 (MCGF2); B cell stimulating factor 1 (BSF-1) |
| Comments： | This is a cloned T cell line derived from C57BL/6TL+ spleen cells. The cells produce IL-3, TCGF2, MCGF2 and BSF-1. |
| Complete Growth Medium： | RPMI 1640 with 2.0 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; fetal bovine serum, 10%; rat T-STIM with Con A, 10%.
Rat T-STIM with Con A can be obtained from Becton Dickinson, Catalogue No. 354115
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| Subculturing： | Medium Renewal: Every 2 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 0.5 X 10 exp5 and 3 X 10 exp6 cells/ml. |
| Cryopreservation： | RPMI 1640 medium 75%; fetal bovine serum, 20%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | Dana Farber Cancer Inst. |
| Deposited As： | Mus musculus |
| U.S. Patent Number： | |
| References： | Cantor HI, Nabel G. Lymphocyte growth factor. US Patent 4,613,459 dated Sep 23 1986 Nabel G, et al. Use of cloned populations of mouse lymphocytes to analyze cellular differentiation. Cell 23: 19-28, 1981. PubMed: 6971154 Nabel G, et al. Multiple biologic activities of a cloned inducer T-cell population. Proc. Natl. Acad. Sci. USA 78: 1157-1161, 1981. PubMed: 6785749 Mosmann TR, et al. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J. Immunol. 136: 2348-2357, 1986. PubMed: 2419430 |
| Cross References： | Nucleotide (GenBank) : E00420 cDNA sequence encoding peptide having MCGF and multi-CSF activity. |