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微信小章| 产品名称： | CIA-E-7.12 |
|---|---|
| 商品货号： | TS212355 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with human epsilon heavy chain. |
| Derivation： | Lymph node cells were fused with S194/5.XXO.BU.1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human epsilon immunoglobulin heavy chain (IgE) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human epsilon immunoglobulin heavy chain (IgE) |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in pristane-primed BALB/c mice (Generally, the first i.p. passage yields mostly adherent tumor cells and little ascites, but serial passage of the ascites cell in additional BALB/c mice will yield large amounts of ascites that may contain up to 20 mg/ml of the antibody. For monoclonal antibody production in ascites, cells should be injected (10 to 20 million cells) intraperitoneally (i.p.) in pristane-primed BALB/c mice.) |
| Comments： | Animals were immunized with a human IgE paraprotein (PS). Lymph node cells were fused with S194/5.XXO.BU.1 myeloma cells. The antibody reacts with human epsilon heavy chain. It has a very high affinity (9 X 10 exp-10 m/L) for an epitope located between amino acids 218 and 301 (CH2 domain) of human epsilon. It does not bind to protein A, but functions well in histamine release assays. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | A Saxon |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Macy E, et al. Enhanced ELISA: how to measure less than 10 picograms of a specific protein (immunoglobulin) in less than 8 hours. FASEB J. 2: 3003-3009, 1988. PubMed: 3263291 Hassner A, Saxon A. Isotype-specific human suppressor T cells for IgE synthesis activated by IgE-anti-IgE immune complexes. J. Immunol. 132: 2844-2849, 1984. PubMed: 6233363 Generally, the first i.p. passage yields mostly adherent tumor cells and little ascites, but serial passage of the ascites cell in additional BALB/c mice will yield large amounts of ascites that may contain up to 20 mg/ml of the antibody. For monoclonal antibody production in ascites, cells should be injected (10 to 20 million cells) intraperitoneally (i.p.) in pristane-primed BALB/c mice. |