微信小陈
微信小章| 产品名称： | CHO-ICAM-1 |
|---|---|
| 商品货号： | TS212358 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | ovary |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent, adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Applications： | The cells can be used in adherence assays as a target cell for malaria infected erythrocytes. Functional expression of the transfected receptor molecules on CHO-ICAM-1 was confirmed using parasites with different cytoadherence phenotypes and cytoadherence inhibition experiments with a panel of anti ICAM-1 antibodies. The use of isolates obtained from malaria patients in The Gambia confirmed the applicability of using these cells in assays for laboratory studies. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The CHO-ICAM-1 cell line was constructed at DNAX Research Institute, Palo Alto, California by transfecting Chinese hamster ovary (CHO) cells with linearized cDNA of human ICAM-1 in pCDM8 by electroporation in a 10 fold molar excess over pBJ1-Neo. The use of isolates obtained from malaria patients in The Gambia confirmed the applicability of using these cells in assays for laboratory studies. |
| Clinical Data： | The use of isolates obtained from malaria patients in The Gambia confirmed the applicability of using these cells in assays for laboratory studies. female |
| Comments： | The CHO-ICAM-1 cell line was constructed at DNAX Research Institute, Palo Alto, California by transfecting Chinese hamster ovary (CHO) cells with linearized cDNA of human ICAM-1 in pCDM8 by electroporation in a 10 fold molar excess over pBJ1-Neo. Stable transfectants were then selected by growth in culture medium containing G418. CHO-ICAM-1 cells express high levels of human ICAM-1 (a ligand for erythrocytes infected with the human malaria parasite, Plasmodium falciparum). The cells can be used in adherence assays as a target cell for malaria infected erythrocytes. Functional expression of the transfected receptor molecules on CHO-ICAM-1 was confirmed using parasites with different cytoadherence phenotypes and cytoadherence inhibition experiments with a panel of anti ICAM-1 antibodies. The use of isolates obtained from malaria patients in The Gambia confirmed the applicability of using these cells in assays for laboratory studies. A culture submitted to the ATCC in August 1993 was found to be contaminated with mycoplasma, and the line was cured by a 21 day treatment with BM Cycline. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:6 to 1:10 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | BL Pasloske |
| Deposited As： | Cricetulus griseus |
| References： | Hasler T, et al. An improved microassay for Plasmodium falciparum cytoadherence using stable transformants of Chinese hamster ovary cells expressing CD36 or intercellular adhesion molecule-1. Am. J. Trop. Med. Hyg. 48: 332-347, 1993. PubMed: 7682381 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |