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| 产品名称： | CHO-CD36 |
|---|---|
| 商品货号： | TS212363 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | ovary |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Applications： | The cells can be used in adherence assays as a target cell for malaria infected erythrocytes. Functional expression of the transfected receptor molecules on CHO-CD36 was confirmed using parasites with different cytoadherence phenotypes and cytoadherence inhibition experiments with a panel of anti-CD36 antibodies. The use of isolates obtained from malaria patients in The Gambia confirmed the applicability of using these cells in assays for laboratory studies. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The CHO-CD36 cell line was constructed at DNAX Research Institute, Palo Alto, California by transfecting Chinese hamster ovary (CHO) cells with linearized cDNA of human CD36 in pCDM8 by electroporation in a 10 fold molar excess over pBJ1-Neo. The use of isolates obtained from malaria patients in The Gambia confirmed the applicability of using these cells in assays for laboratory studies. |
| Clinical Data： | The use of isolates obtained from malaria patients in The Gambia confirmed the applicability of using these cells in assays for laboratory studies. female |
| Comments： | The CHO-CD36 cell line was constructed at DNAX Research Institute, Palo Alto, California by transfecting Chinese hamster ovary (CHO) cells with linearized cDNA of human CD36 in pCDM8 by electroporation in a 10 fold molar excess over pBJ1-Neo. Stable transfectants were then selected by growth in culture medium containing G418. CHO-CD36 cells express high levels of human CD36 (a ligand for erythrocytes infected with the human malaria parasite, Plasmodium falciparum). The cells can be used in adherence assays as a target cell for malaria infected erythrocytes. Functional expression of the transfected receptor molecules on CHO-CD36 was confirmed using parasites with different cytoadherence phenotypes and cytoadherence inhibition experiments with a panel of anti-CD36 antibodies. The use of isolates obtained from malaria patients in The Gambia confirmed the applicability of using these cells in assays for laboratory studies. A culture submitted to the ATCC in August 1993 was found to be contaminated with mycoplasma, and the line was cured by a 21 day treatment with BM Cycline. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:6 to 1:10 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published byxa0Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |