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| 产品名称： | CHP-212 |
|---|---|
| 商品货号： | TS212367 |
| Organism： | Homo sapiens, human |
| Tissue： | brain |
| Product Format： | frozen |
| Morphology： | neuroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | neuroblastoma |
| Storage Conditions： | liquid nitrogen vapor phase |
| Complete Growth Medium： | The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:10 to 1:50 every 2 toxa03 weeksxa0 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
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| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 12 D13S317: 8,13 D16S539: 13 D5S818: 10,12 D7S820: 11 THO1: 6 TPOX: 8,11 vWA: 15,18 |
| Name of Depositor： | JL Biedler |
| Deposited As： | Homo sapiens |