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购买须知
| 产品名称： | CH1 |
|---|---|
| 商品货号： | TS212369 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | B10.H-2aH-4bp/Wts(2a4b) |
| Applications： | transfection host |
| Derivation： | This line was established in tissue culture from the CH1 B cell lymphoma. |
| Antigen Expression： | I-Ak; I-Ek |
| Genes Expressed： | immunoglobulin (surface); against E. coli,I-Ak; I-Ek |
| Cellular Products： | immunoglobulin (surface); against E. coli |
| Comments： | This line was established in tissue culture from the CH1 B cell lymphoma. The cells produce, but do not secrete, immunoglobulin. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp4 cells/ml and maintain between 3 x 10 exp5 and 8 x 10 exp5 cells/ml. |
| Isotype： | IgM; lambda light chain |
| Name of Depositor： | G Haughton |
| Deposited As： | Mus musculus |
| References： | Lanier LL, et al. Transplantable B-cell lymphomas in B10. H-2aH-4bp/Wts mice. Immunogenetics 16: 367-371, 1982. PubMed: 6983495 Lanier LL, et al. Novel type of murine B-cell lymphoma. Nature 271: 554-555, 1978. PubMed: 304532 J. Immunol. 121: 2353, 1978. |