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微信小章| 产品名称： | ChaGo-K-1 |
|---|---|
| 商品货号： | TS212374 |
| Organism： | Homo sapiens, human |
| Tissue： | lung,xa0bronchus |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | bronchogenic carcinoma |
| Age： | 45 years |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a hyperdiploid human cell line. The modal chromosome number is 52, occurring at 40% with polyploidy at 3.8%., Over 2/3 of the chromosome complement consist of those with structural rearrangements (or markers) including 1q, t(1p,16q), del(3)(p13), t(5;14)(q35;q11), t(9q;?)1, t(9q;?)2, 12p+, 20p+, t(Xq,7p)., Twenty-one other markers are common to most cells. The normal chromosomes 1,3,5,9,14,15 and X are absent. All three E group chromosomes are paired., Y chromosome not detected by Q staining. |
| Genes Expressed： | hCG; human chorionic gonadotropin (alpha subunit only); estradiol; progesterone; mucin (apomucin, MUC-1, MUC-2) |
| Cellular Products： | hCG; human chorionic gonadotropin (alpha subunit only); estradiol; progesterone; mucin (apomucin, MUC-1, MUC-2) |
| Comments： | The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: 5% CO2xa0 |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 8,12 THO1: 9.3 TPOX: 8 vWA: 17,18 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 LDH, 5 Me-2, 0 PGM1, 1 PGM3, 1 |
| Name of Depositor： | S Lippman, A Rabson |
| Deposited As： | Homo sapiens |
| References： | Lieblich JM, et al. Ectopic and eutopic secretion of chorionic gonadotropin and its subunits in vitro: Comparison of clonal strains from carcinomas of lung and placenta. J. Natl. Cancer Inst. 56: 911-917, 1976. PubMed: 62839 Rabson AS, et al. Production of human chorionic gonadotropin in vitro by a cell line derived from a carcinoma of the lung. J. Natl. Cancer Inst. 50: 669-674, 1973. PubMed: 4708151 Dahiya R, et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993. PubMed: 8443822 Tashjian AH Jr., et al. Subunits of human chorionic gonadotropin: unbalanced synthesis and secretion by clonal cell strains derived from a bronchogenic carcinoma. Proc. Natl. Acad. Sci. USA 70: 1419-1422, 1973. PubMed: 4514312 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |