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| 产品名称： | CHL/IU CHL-11 |
|---|---|
| 商品货号： | TS212376 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | lung |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | newborn |
| Gender： | female |
| Applications： | The line is useful for screening chemical for ability to induce chromosomal aberrations. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 25 |
| Derivation： | This line was derived from the CHL line established by Utakoji in 1970. |
| Complete Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; bovine calf serum, 10%
|
| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: 1 to 2 times per week |
| Cryopreservation： | Complete growth medium supplemented with 5% DMSO |
| Culture Conditions： | Temperature: 37°C |
| Population Doubling Time： | 15 hrs |
| Name of Depositor： | M Ishidate |
| Deposited As： | Cricetulus griseus |
| Year of Origin： | 1970 |
| References： | Data book of chromosomal aberration test in vitro. New York: Elsevier; 1988. Ishidate M Jr., Odashima S. Chromosome tests with 134 compounds on Chinese hamster cells in vitro-- a screening for chemical carcinogens. Mutat. Res. 48: 337-354, 1977. PubMed: 876270 Koyama H, et al. A new cell line derived from newborn Chinese hamster lung tissue. Gann 61: 161-167, 1970. PubMed: 5452405 |