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微信小章| 产品名称： | Cf2Th |
|---|---|
| 商品货号： | TS212379 |
| Organism： | Canis familiaris, dog |
| Tissue： | thymus |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | newborn |
| Gender： | female |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Cf2Th was derived from thymus tissue of newborn dog |
| Comments： | The line has been used to propagate retroviruses from cat embryo and baboon lung and kidney. The cells are also susceptible to xenotropic retroviruses from AKR and BALB/c mice. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with non-essential amino acids, 80%; fetal bovine serum, 20%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete culture medium described above supplemented with 5% (v/v) DMSO.xa0xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Name of Depositor： | WA Nelson-Rees |
| Deposited As： | Canis familiaris |
| References： | Nelson-Rees WA, et al. Source, alterations, characteristics and use of a new dog cell line (Cf2Th). In Vitro 12: 665-669, 1976. PubMed: 190163 Farzan M, et al. HIV-1 Entry and Macrophage Inflammatory Protein-1beta-mediated Signaling Are Independent Functions of the Chemokine Receptor CCR5. J. Biol. Chem. 272: 6854-6857, 1997. PubMed: 9054370 Campbell M, et al. The simian foamy virus type 1 transcriptional transactivator (Tas) binds and activates an enhancer element in the gag gene. J. Virol. 70: 6847-6855, 1996. PubMed: 8794326 Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |