微信小陈
微信小章
产品简介
购买须知
| 产品名称： | CDR2 |
|---|---|
| 商品货号： | TS212388 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The primary specificity is for the thymic cortex, although there is faint non-epithelial reactivity in the medulla. It cross-reacts with epithelial elements in human kidney, tonsils, pancreatic ducts, lymph nodes and esophagus. |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human cortical thymic epithelium |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human cortical thymic epithelium |
| Comments： | Animals were immunized with homogenized human thymus (1.5 to 2 years of age). Spleen cells were fused with Sp2/0 myeloma cells. The primary specificity is for the thymic cortex, although there is faint non-epithelial reactivity in the medulla. The antibody does not react with mouse or rat tissues. It cross-reacts with epithelial elements in human kidney, tonsils, pancreatic ducts, lymph nodes and esophagus. |
| Complete Growth Medium： | Iscoves Modified Dulbeccos Medium (ATCC® 30-2005), 90%; fetal bovine serum (ATCC® 30-2020), 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2b |
| Name of Depositor： | RV Rouse |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413 |