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微信小章| 产品名称： | CCRF-SB CCRF SB |
|---|---|
| 商品货号： | TS212394 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells contain Herpesvirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute lymphoblastic leukemia |
| Age： | 11.5 years |
| Gender： | Male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Derived from a buffy coat preparation from a patient with acute lymphoblastic leukemia. A T cell line (HSB-2, TS212394.1) was also obtained from the same buffy coat preparation. |
| Clinical Data： | 11.5 years
Caucasian
male |
| Antigen Expression： | HLA A1, A2, B12, B17, Cw2 |
| Comments： | The cells are EBNA positive |
| Complete Growth Medium： | RPMI 1640 medium, 90%; fetal bovine serum, 10% - OR - Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10%
|
| Subculturing： | Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 X 105 viable cells/mL.xa0
Medium Renewal: Add fresh medium (10% to 20% by volume) every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 10,12 D16S539: 9,13 D5S818: 11,12 D7S820: 11,12 THO1: 9,10 TPOX: 8 vWA: 18 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | GE Foley |
| Deposited As： | Homo sapiens |
| References： | Foley GE et al. M.D. Anderson Hospital and Tumor Inst. Monograph 21, 1967. Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381 Santra M, et al. Ectopic expression of decorin protein core causes a generalized growth suppression in neoplastic cells of various histogenetic origin and requires endogenous p21, an inhibitor of cyclin-dependent kinases. J. Clin. Invest. 100: 149-157, 1997. PubMed: 9202067 Qian JX, et al. Decreased interleukin-15 from activated cord versus adult peripheral blood mononuclear cells and the effect of interleukin-15 in upregulating antitumor immune activity and cytokine production in cord blood. Blood 90: 3106-3117, 1997. PubMed: 9376592 |