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| 产品名称： | CEM-CM3 |
|---|---|
| 商品货号： | TS212395 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | T lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute lymphoblastic leukemia |
| Age： | 4 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | This line was derived from CCRF-CEM (see ATCC CCL-119) by selecting for resistance to 8-azaguanine. The line has been used as a fusion partner in the production of human-human T cell hybridomas. |
| Storage Conditions： | liquid nitogen vapor phase |
| Derivation： | This line was derived from CCRF-CEM (see ATCC CCL-119) by selecting for resistance to 8-azaguanine. |
| Clinical Data： | female Caucasian 4 years |
| Comments： | This line was derived from CCRF-CEM (see ATCC CCL-119) by selecting for resistance to 8-azaguanine. The line has been used as a fusion partner in the production of human-human T cell hybridomas. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.015 mg/ml 8-azaguanine, 90%; fetal bovine serum, 10%
|
| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: culture medium 95%; DMSO, 5% Storage temperature: liquid nitogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 12 D16S539: 10,12 D5S818: 12,13 D7S820: 10,13 THO1: 6,7 TPOX: 8 vWA: 18,19 |
| Name of Depositor： | MK Cathcart |
| Deposited As： | Homo sapiens |
| References： | Cathcart MK, Murakami MNonspecific suppression of antibody synthesis by products of human T lymphocyte hybridomas. Use of a newly selected parent fusion lineIn: Cathcart MK, Murakami MT cell hybridomasBoca Raton, FLCRC Presspp. 227-236, 1985 . Human T lymphocyte hybridomas: production of nonspecific suppressor factors of antibody synthesis. Kyoto, Japan: 5th International Congress of Immunology; 1983. . . Clin. Res. 31: 734A, 1983. |