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| 产品名称： | CCD-986Sk |
|---|---|
| 商品货号： | TS212404 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 22 years |
| Gender： | female |
| Ethnicity： | Black |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established from skin taken from normal breast tissue removed during breast reduction mammoplasty. |
| Clinical Data： | 22 years Black female |
| Comments： | The cells have been observed to senesce at about 36 population doublings. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze Medium: Complete growth medium 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 11,13 D13S317: 9,12 D16S539: 8 D5S818: 11,13 D7S820: 10,11 THO1: 7,9.3 TPOX: 8,11 vWA: 14,17 |