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| 产品名称： | CCRF S-180 II |
|---|---|
| 商品货号： | TS212405 |
| Organism： | Mus musculus, mouse |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | sarcoma |
| Age： | adult |
| Strain： | CFW |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Tumorigenic： | Yes |
| Effects： | Yes, in CFW mice |
| Virus Resistance： | poliovirus 1 |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Minimum essential medium (Eagle) in Earles BSS with non-essential amino acids, 95%; bovine calf serum, 5%
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution for 2 to 5 minutes, remove trypsin and add fresh medium. Aspirate cells and dispense into new flasks. |
| Name of Depositor： | GE Foley |
| Deposited As： | Mus musculus |
| References： | Foley GE, et al. Isolation and serial propagation of malignant and normal cells in semi-defined media. Origins of CCRF cell lines. Cancer Res. 20: 930-939, 1960. PubMed: 13823721 Foley EG, Drolet BP. Sustained propagation of sarcoma of 180 in tissue culture. Proc. Soc. Exp. Biol. Med. 92: 347-352, 1956. PubMed: 13350343 |