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微信小章| 产品名称： | CCRF-HSB-2 CCRF HSB-2, HSB-2 |
|---|---|
| 商品货号： | TS212407 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | T lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute lymphoblastic leukemia |
| Age： | 11.5 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Derived from the same buffy coat preparation as CCL-120 (CCRF-SB) by serially transplanting into newborn syrian hamsters. |
| Clinical Data： | 11.5 years Caucasian male |
| Antigen Expression： | HLA A1, A2, B12, B17, Cw2; CD5 (78%), CD7 (96%) |
| Tumorigenic： | Yes, in Syrian hamsters |
| Effects： | Yes, in Syrian hamsters |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3xa0to 5xa0x 105 viable cells/mL. A maximum of 2xa0to 3xa0x 106 viable cells/mL is obtainable. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 10,12 D16S539: 9,13 D5S818: 11,12 D7S820: 11,12 THO1: 9,10 TPOX: 8 vWA: 18,19 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | GE Foley |
| Deposited As： | Homo sapiens |
| References： | Foley GE et al. M.D. Anderson Hospital and Tumor Inst. Monograph 21, 1967. Adams RA, et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121-1125, 1968. PubMed: 4872716 Proc. Am. Assoc. Cancer Res. 8: 1, 1967. |
| Cross References： | Nucleotide (GenBank) : NM_004429 Homo sapiens ephrin-B1 (EFNB1), mRNA. Nucleotide (GenBank) : U09303 Human T cell leukemia LERK-2 (EPLG2) mRNA, complete cds. Nucleotide (GenBank) : NM_004429 Homo sapiens ephrin-B1 (EFNB1), mRNA. Nucleotide (GenBank) : U09303 Human T cell leukemia LERK-2 (EPLG2) mRNA, complete cds. |