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| 产品名称： | CCD-29Lu |
|---|---|
| 商品货号： | TS212409 |
| Organism： | Homo sapiens, human |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 80 years |
| Gender： | male |
| Ethnicity： | Black |
| Clinical Data： | male Black 80 years |
| Comments： | The line is at population doubling level (PDL) = 12, and begins to senesce at about PDL = 24. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |