微信小陈
微信小章
产品简介
购买须知
| 产品名称： | CCD-19Lu | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 商品货号： | TS212413 | |||||||||
| Organism： | Homo sapiens, human | |||||||||
| Tissue： | lung | |||||||||
| Cell Type： | fibroblast | |||||||||
| Product Format： | frozen | |||||||||
| Morphology： | fibroblast | |||||||||
| Culture Properties： | adherent | |||||||||
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
|||||||||
| Disease： | normal | |||||||||
| Age： | 20 years | |||||||||
| Gender： | female | |||||||||
| Ethnicity： | Caucasian | |||||||||
| Storage Conditions： | liquid nitrogen vapor phase | |||||||||
| Karyotype： | normal female; diploid; stable | |||||||||
| Derivation： | The line was derived from normal tissue from patient who died of trauma to the head. |
|||||||||
| Clinical Data： | 20 years
Caucasian
female
|
|||||||||
| Comments： | The cells are capable of at least 8 or 9 population doublings beyond the current freeze. |
|||||||||
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
|||||||||
| Subculturing： |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Twice per week
|
|||||||||
| Cryopreservation： |
|