微信小陈
微信小章
产品简介
购买须知
| 产品名称： | CCD-1139Sk |
|---|---|
| 商品货号： | TS212422 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 62 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | male Caucasian 62 years |
| Comments： | Cells senesce after approximately 52 population doublings. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: 1:2 to 1:6 is recommened Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phaseliquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | P.Wood, ATCC |
| Deposited As： | human |