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| 产品名称： | CCD-1099Sk |
|---|---|
| 商品货号： | TS212440 |
| Organism： | Homo sapiens, human |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 24 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | The line was established from normal skin from the leg. |
| Derivation： | The line was established from normal skin from the leg. |
| Clinical Data： | male Caucasian 24 years |
| Comments： | The line was established from normal skin from the leg. Cells senesce after approximately 60 population doublings. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Twice per week Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | H.White, ATCC |