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| 产品名称： | CCD-1095Sk |
|---|---|
| 商品货号： | TS212449 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Carcinoma |
| Age： | 37 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | The line was established from normal skin taken at biopsy from the left breast. |
| Storage Conditions： | liquid nitorgen vapor phase |
| Derivation： | The line was established from normal skin taken at biopsy from the left breast. |
| Clinical Data： | The patient had infiltrating ductal carcinoma with associated Pagets disease of the nipple. female Caucasian 37 years |
| Comments： | The line was established from normal skin taken at biopsy from the left breast. The patient had infiltrating ductal carcinoma with associated Pagets disease of the nipple. The cells senesce after approximately 36 population doublings. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |