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| 产品名称： | CCD-1087Sk |
|---|---|
| 商品货号： | TS212450 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 18 years |
| Gender： | female |
| Applications： | The line was established from normal skin from the breast of a patient undergoing reduction mammoplasty. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established from normal skin from the breast of a patient undergoing reduction mammoplasty. |
| Clinical Data： | The line was established from normal skin from the breast of a patient undergoing reduction mammoplasty. The patient had interstitial fibrosis and hyalinization consistent with macromastia. female |
| Comments： | The line was established from normal skin from the breast of a patient undergoing reduction mammoplasty. The patient had interstitial fibrosis and hyalinization consistent with macromastia. The cells senesce after approximately 43 population doublings. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 3 to 4 days Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |