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| 产品名称： | CCD-1079Sk |
|---|---|
| 商品货号： | TS212451 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Age： | newborn |
| Gender： | male |
| Derivation： | The line was established from skin taken from normal foreskin. |
| Clinical Data： | male
newborn |
| Comments： | The cells senesce after approximately 56 population doublings. |
| Complete Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%, fetal bovine serum, 10%
|
| Subculturing： | Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 3 to 4 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 12,13 D13S317: 11,12 D16S539: 9,11 D5S818: 11,12 D7S820: 12 THO1: 6,9.3 TPOX: 10,11 vWA: 17,18 |